Services

Microscopes & Equipment

Olympus IXplore SpinSR10

The Olympus IXplore SpinSR10 spinning disk confocal super resolution microscope is designed to provide super resolution (down to 120nm XY resolution) and prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching.

The microscope has been adapted with a Rapp OptoElectronic UGA-42 Caliburn 355/42 pulsed laser (355nm, 1KHz, 42µJ/pulse) to perform ablation and microdissection.

Objectives

4x/0.16 UPLXAPO | Air | WD: 13mm

10x/0.40 UPLXAPO | Air | WD: 3.1mm

20x/0.80 UPLXAPO | Air | WD: 0.6mm

40x/1.30 UPLFLN | • Oil | WD: 0.2mm

60x/1.42 UPLXAPO | • Oil | WD: 0.15mm

100x/1.30 UPLFLN | • Oil | WD: 0.2mm

100x/1.50 UPLAPO | • Oil | WD: 0.12mm

Camera

HAMAMATSU ORCA FusionBT

Laser Lines

405 nm | Diode | 50mW

488 nm | Diode | 100mW

561 nm | Diode | 100mW

640 nm | Diode | 100mW

Filters

Ex: Multi-band 405 - 488 - 561 - 640

Em: Band-pass 447/60 | 525/50 | 617/73 | 685/40

Leica TCS SP5 + MultiPhoton

The Leica TCS SP5 is an advanced confocal microscope suitable for scanning fixed samples and live cell imaging.

The microscope is equipped with 3 PMT, 2 Hybrid and 2 APD detectors and has been adapted with a Mai-Tai DeepSee Infrared Laser to perform Multiphoton Microscopy and with a Becker & Hickl Simple-Tau 830 TCSPC system to carry out FLIM, FCS and FCCS experiments.

Objectives

10x/0.22 HI PL I Ph1 | Air | WD: 7.8mm

10x/0.30 HC PL FLUOTAR | Air | WD: 11mm

20x/0.30 HI PL I Ph1 | Air | WD: 3mm

20x/0.70 HC PL APO CS | Air | WD: 0.59mm

40x/0.60 HCX PL FL L CORR | Air | 3.3-1.9mm

40x/0.85 HCX PL APO CS CORR | Air | WD: 0.4mm

63x/1.20 W HCX PL APO CS CORR | Water | WD: 0.22mm

63x/1.30 Glyc HCX PL APO CS | Glyc | WD: 0.28mm

100x/1.30 Oil HCX PL FLUOTAR | • Oil | WD: 0.13mm

Detectors

3 PMT

2 GaAsP Hybrid Detectors

2 APD

Laser Lines

405 nm | Pulsed Diode | 3mW (avg.)

458 nm | Argon | 65mW

476 nm | Argon | 65mW

488 nm | Argon | 65mW

496 nm | Argon | 65mW

514 nm | Argon | 65mW

543 nm | HeNe | 5mW

633 nm | HeNe | 10mW

690-1040nm | Ti:Sapphire | 2.4W (avg.)

Filters

Acousto Optical Beam Splitter (AOBS)

Zeiss Axio Observer 7 - Apotome 3

The Zeiss Axio Observer 7 is a standard widefield fluorescence microscope equipped with a Zeiss Apotome 3, capable of producing optical sections of a fluorescent specimen by removing the out-of-focus light using structured Illumination.

Objectives

10x/0.25 LD A-Plan Ph1 | Air | WD: 8.5mm

20x/0.40 LD Plan-Neofluar Corr Ph2 | Air | WD: 7.4-8.4mm

20x/0.80 PL APO | Air | WD: 0.55mm

40x/0.55 LD A-Plan Ph2 | Air | WD: 2mm

63x/1.40 Oil PL APO | Oil | WD: 0.19mm

Camera

Axiocam 503 Mono

Light Source

Colibri 5 RGB-UV LED

385 nm | 475 nm | 555 nm | 630 nm

Filters

Ex: BP 370-410 | BS: 420 | Em: BP 430-470

Ex: BP 450-490 | BS: 495 | Em: BP 500-550

Ex: BP 538-562 | BS: 570 | Em: BP 570-640

Ex: BP 625-655 | BS: 660 | Em: BP 665-715

Nikon C1si Confocal Scanning Microscope

The Nikon Eclipse TE-2000 with the C1si confocal module is a standard confocal microscope, capable of scanning fixed specimen and live cell imaging. The Nikon C1si is capable to acquire 32 channel spectral images over a range of 320nm at 10nm spectral resolution making possible the high data acquisition rates necessary for live cell spectral imaging.

Objectives

20x/0.40 Nikon LWD | Air | WD: 3mm

40x/1.30 Oil S Fluor | Oil | WD: 0.22mm

60x/1.49 Oil Plan APO TIRF | Oil | WD: 0.12mm

Detectors

3 PMT

32 channel Spectral detector

Laser Lines

405 nm | Diode | 160mW

458 nm | Argon | 150mW

476 nm | Argon | 150mW

488 nm | Argon | 150mW

496 nm | Argon | 150mW

514 nm | Argon | 150mW

561 nm | Diode | 100mW

637 nm | Diode | 100mW

Filters

PMT 1:

em: BS 506 | BP 485/40

em: BS 540 | BP 515/30

PMT 3 >> PMT 2:

em: BS 458 | BP 438/24 >> BP 483/32

em: BS 570 | BP 535/50 >> LP 610

em: BS 625 | BP 593/40 >> LP 650

JPK Nanowizard UltraSpeed AFM

Mounted on a Leica DM 4000 widefield fluorescence microscope, the JPK Nanowizard UltraSpeed Atomic Force Microscope (AFM) enables the tracking of changes in the specimen in real time.

Objectives

10x/0.22 HI PL I Ph1 | Air | WD: 7.8mm

10x/0.30 HC PL FLUOTAR | Air | WD: 11mm

20x/0.30 HI PL I Ph1 | Air | WD: 3mm

20x/0.70 HC PL APO CS | Air | WD: 0.59mm

40x/0.60 HCX PL FL L CORR | Air | 3.3-1.9mm

40x/0.85 HCX PL APO CS CORR | Air | WD: 0.4mm

63x/1.20 W HCX PL APO CS CORR | Water | WD: 0.22mm

63x/1.30 Glyc HCX PL APO CS | Glyc | WD: 0.28mm

100x/1.30 Oil HCX PL FLUOTAR | • Oil | WD: 0.13mm

Light Source

Leica EL6000

Filters

Ex: BP 436/20 | BS: 455 | Em: BP 480/40

Ex: BP 470/40 | BS: 510 | Em: LP 515

Ex: BP 545/30 | BS: 565 | Em: BP 610/75

Ex: BP 620/60 | BS: 660 | Em: BP 700/75

Camera

Hamamatsu ORCA-R2


Light Sheet Microscope

Coming Soon

ZEISS Microscopy, CC BY 2.0 <https://creativecommons.org/licenses/by/2.0>, via Wikimedia Commons

Image Analysis Workstation Pro

CPU: Intel Xeon W-2255 3.70GHz (10 Cores)

RAM: 512GB

GPU: Nvidia RTX A4000 16GB

Software: Bitplane Imaris 9.7 Core Facility, Huygens Essentials 21.04, Matlab R2021a, FiJi/ImageJ, QuPath, Icy, Ilastik, CellProfiler, Leica LAS X Core, Nikon NIS-Elements viewer, Zeiss Zen Lite, FLIMFit, SPCImage, Agave 3D, Blender...

Image Analysis Workstation Lite

CPU: Intel Xeon E5-1650 3.2Ghz (6 Cores)

RAM: 32GB

GPU: Nvidia GTX970 4GB

Software: Matlab R2017b, FiJi/ImageJ, QuPath, Icy, Ilastik, CellProfiler, Leica LAS X Core, Nikon NIS-Elements viewer, Zeiss Zen Lite, FLIMFit, SPCImage, Agave 3D, Blender...