Services
Microscopes & Equipment
Super Resolution | Confocal | Two-Photon | Structured Illumination | AFM | Micropatterning | Light Sheet | Image Analysis
- Super Resolution
Olympus IXplore SpinSR10
The Olympus IXplore SpinSR10 spinning disk confocal super resolution microscope is designed to provide super resolution (down to 120nm XY resolution) and prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching.
The microscope has been adapted with a Rapp OptoElectronic UGA-42 Caliburn 355/42 pulsed laser (355nm, 1kHz, 42µJ/pulse) to perform ablation and microdissection.
Objectives
• 4x/0.16 UPLXAPO | Air | WD: 13mm
• 10x/0.40 UPLXAPO | Air | WD: 3.1mm
• 20x/0.80 UPLXAPO | Air | WD: 0.6mm
• 40x/1.30 UPLFLN | • Oil | WD: 0.2mm
• 60x/1.42 UPLXAPO | • Oil | WD: 0.15mm
• 100x/1.30 UPLFLN | • Oil | WD: 0.2mm
• 100x/1.50 UPLAPO | • Oil | WD: 0.12mm
Camera
· Resolution 2304px x 2304px
· Pixel Size: 6.5µm x 6.5µm
· Effective Area: 14.976mm × 14.976mm
· Bit depth: 16bit
Laser Lines
405 nm | Diode | 50mW
488 nm | Diode | 100mW
561 nm | Diode | 100mW
640 nm | Diode | 100mW
Filters
Ex: Multi-band 405 - 488 - 561 - 640
Em: Band-pass 447/60 | 525/50 | 617/73 | 685/40
Manuals 🔗
- Confocal & Two-Photon
Leica Confocal TCS SP5 + Two-Photon
The Leica TCS SP5 is an advanced confocal microscope suitable for scanning fixed samples and live cell imaging.
The microscope is equipped with 3 PMT, 2 Hybrid and 2 APD detectors and has been adapted with a Mai-Tai DeepSee Infrared Laser to perform Multiphoton Microscopy and with a Becker & Hickl Simple-Tau 830 TCSPC system to carry out FLIM, FCS and FCCS experiments.
Objectives
• 10x/0.22 HI PL I Ph1 | Air | WD: 7.8mm
• 10x/0.30 HC PL FLUOTAR | Air | WD: 11mm
• 20x/0.30 HI PL I Ph1 | Air | WD: 3mm
• 20x/0.70 HC PL APO CS | Air | WD: 0.59mm
• 40x/0.60 HCX PL FL L CORR | Air | WD: 3.3-1.9mm
• 40x/0.75 HCX PL APO U-V-I | Air | WD: 0.28mm
• 40x/0.85 HCX PL APO CS CORR | Air | WD: 0.24mm
• 63x/1.20 W HCX PL APO CS CORR | • Water | WD: 0.22mm
• 63x/1.30 Glyc HCX PL APO CS | • Glyc | WD: 0.28mm
• 100x/1.30 Oil HCX PL FLUOTAR | • Oil | WD: 0.13mm
Detectors
3 PMT
2 GaAsP Hybrid Detectors
2 APD
Laser Lines
405 nm | Pulsed Diode | 3mW (avg.)
458 nm | Argon | 65mW
476 nm | Argon | 65mW
488 nm | Argon | 65mW
496 nm | Argon | 65mW
514 nm | Argon | 65mW
543 nm | HeNe | 5mW
633 nm | HeNe | 10mW
690-1040nm | Ti:Sapphire | 2.4W (avg.)
Filters
Acousto Optical Beam Splitter (AOBS)
Manuals 🔗
Nikon C1si Confocal Scanning Microscope
The Nikon Eclipse TE-2000 with the C1si confocal module is a standard confocal microscope, capable of scanning fixed specimen and live cell imaging. The Nikon C1si is capable to acquire 32 channel spectral images over a range of 320nm at 10nm spectral resolution making possible the high data acquisition rates necessary for live cell spectral imaging.
Objectives
• 10x/0.25 CFI Plan Fluor | Air | WD: 16mm
• 20x/0.40 LWD | Air | WD: 3mm
• 20x/0.45 ELWD S Plan Fluor | Air | WD: 8.2-6.9mm
• 40x/1.30 Oil S Fluor | • Oil | WD: 0.22mm
• 60x/1.49 Oil Plan APO TIRF | • Oil | WD: 0.12mm
Detectors
3 PMT
32 channel Spectral detector
Manuals 🔗
Laser Lines
405 nm | Diode | 160mW
458 nm | Argon | 150mW
476 nm | Argon | 150mW
488 nm | Argon | 150mW
496 nm | Argon | 150mW
514 nm | Argon | 150mW
561 nm | Diode | 100mW
637 nm | Diode | 100mW
Filters
PMT 1:
em: BS 506 | BP 485/40
em: BS 540 | BP 515/30
PMT 3 >> PMT 2:
em: BS 458 | BP 438/24 >> BP 483/32
em: BS 570 | BP 535/50 >> LP 610
em: BS 625 | BP 593/40 >> LP 650
- Structured Illumination
Zeiss Axio Observer 7 + Apotome 3
The Zeiss Axio Observer 7 is a standard widefield fluorescence microscope equipped with a Zeiss Apotome 3, capable of producing optical sections of a fluorescent specimen by removing the out-of-focus light using structured Illumination.
Objectives
• 10x/0.25 LD A-Plan Ph1 | Air | WD: 8.5mm
• 20x/0.40 LD Plan-Neofluar Corr Ph2 | Air | WD: 7.4-8.4mm
• 20x/0.80 PL APO | Air | WD: 0.55mm
• 40x/0.55 LD A-Plan Ph2 | Air | WD: 2mm
• 40x/0.95 Plan Apo Corr | Air | WD: 0.25mm
• 63x/1.40 Oil Plan Apo | • Oil | WD: 0.19mm
Camera
· Resolution 1936px x 1460px
· Pixel Size: 4.54µm x 4.54µm
· Effective Area: 8.80mm x 6.60mm
· Bit depth: 14bit
Light Source
385 nm | 475 nm | 555 nm | 630 nm
Filters
Ex: BP 370-410 | BS: 420 | Em: BP 430-470
Ex: BP 450-490 | BS: 495 | Em: BP 500-550
Ex: BP 538-562 | BS: 570 | Em: BP 570-640
Ex: BP 625-655 | BS: 660 | Em: BP 665-715
Manuals 🔗
- Atomic Force Microscopy
JPK Nanowizard UltraSpeed AFM
Mounted on a Zeiss Axio Observer 7 equipped with a Zeiss Apotome 3, the JPK Nanowizard UltraSpeed Atomic Force Microscope (AFM) enables the tracking of changes in the specimen in real time.
Objectives
• 10x/0.25 LD A-Plan Ph1 | Air | WD: 8.5mm
• 20x/0.40 LD Plan-Neofluar Corr Ph2 | Air | WD: 7.4-8.4mm
• 20x/0.80 PL APO | Air | WD: 0.55mm
• 40x/0.55 LD A-Plan Ph2 | Air | WD: 2mm
• 40x/0.95 Plan Apo Corr | Air | WD: 0.25mm
• 63x/1.40 Oil Plan Apo | • Oil | WD: 0.19mm
Camera
· Resolution 2464px × 2056px
· Pixel Size: 3.45µm x 3.45µm
· Effective Area: 8.50mm x 7.10mm
· Bit depth: 14bit
Light Source
385 nm | 475 nm | 555 nm | 630 nm
Filters
Ex: BP 370-410 | BS: 420 | Em: BP 430-470
Ex: BP 450-490 | BS: 495 | Em: BP 500-550
Ex: BP 538-562 | BS: 570 | Em: BP 570-640
Manuals 🔗
- Micropatterning
Alvéole Primo 2 Micropatterning System
Mounted on a Nikon Ti2 widefield fluorescence microscope, the Alvéole Primo 2 allows, all in a single device, micropatterning, hydrogel polymerization and microfabrication, to create bespoke in vitro cellular microenvironments or to get better cell models for cell biology experiments or electron cryotomography studies.
Objectives
• 10x/0.25 CFI Plan Fluor | Air | WD: 16mm
• 20x/0.40 LWD | Air | WD: 3mm
• 20x/0.45 ELWD S Plan Fluor | Air | WD: 8.2-6.9mm
• 40x/1.30 Oil S Fluor | • Oil | WD: 0.22mm
• 60x/1.49 Oil Plan APO TIRF | • Oil | WD: 0.12mm
Camera
Hamamatsu ORCA-R2 (with a 0.7x lens)
· Resolution 1344px x 1024px
· Pixel Size: 6.45µm x 6.45µm
· Effective Area: 8.67mm x 6.60mm
· Bit depth: 12bit
Light Source
Filters
Ex: BP 375/28 | BS: 415 | Em: BP 460/60
Ex: BP 470/40 | BS: 500 | Em: BP 535/50
Ex: BP 540/25 | BS: 565 | Em: BP 605/55
Manuals 🔗
- Light Sheet
Light Sheet Microscope
Coming Soon
- Image Analysis
Image Analysis Workstation Pro
CPU: Intel Xeon W-2255 3.70GHz (10 Cores)
RAM: 512GB
Software: Bitplane Imaris 9.7 Core Facility, Huygens Essentials 21.04, Matlab R2021a, JPK Data Processing, FiJi/ImageJ, QuPath, Icy, Ilastik, CellProfiler, Leica LAS X Core, Nikon NIS-Elements viewer, Zeiss Zen Lite, FLIMFit, SPCImage, Agave 3D, Blender...
Image Analysis Workstation Lite
CPU: Intel Xeon E5-1650 3.2Ghz (6 Cores)
RAM: 32GB
GPU: Nvidia GTX970 4GB
Software: Matlab R2017b, JPK Data Processing, FiJi/ImageJ, QuPath, Icy, Ilastik, CellProfiler, Leica LAS X Core, Nikon NIS-Elements viewer, Zeiss Zen Lite, FLIMFit, SPCImage, Agave 3D, Blender...
