Microscopes & Equipment
The Olympus IXplore SpinSR10 spinning disk confocal super resolution microscope is designed to provide super resolution (down to 120nm XY resolution) and prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching.
The microscope has been adapted with a Rapp OptoElectronic UGA-42 Caliburn 355/42 pulsed laser (355nm, 1KHz, 42µJ/pulse) to perform ablation and microdissection.
The Leica TCS SP5 is an advanced confocal microscope suitable for scanning fixed samples and live cell imaging.
The microscope is equipped with 3 PMT, 2 Hybrid and 2 APD detectors and has been adapted with a Mai-Tai DeepSee Infrared Laser to perform Multiphoton Microscopy and with a Becker & Hickl Simple-Tau 830 TCSPC system to carry out FLIM, FCS and FCCS experiments.
The Nikon Eclipse TE-2000 with the C1si confocal module is a standard confocal microscope, capable of scanning fixed specimen and live cell imaging. The Nikon C1si is capable to acquire 32 channel spectral images over a range of 320nm at 10nm spectral resolution making possible the high data acquisition rates necessary for live cell spectral imaging.
Software: Bitplane Imaris 9.7 Core Facility, Huygens Essentials 21.04, Matlab R2021a, FiJi/ImageJ, QuPath, Icy, Ilastik, CellProfiler, Leica LAS X Core, Nikon NIS-Elements viewer, Zeiss Zen Lite, FLIMFit, SPCImage, Agave 3D, Blender...