About

Members

Facility Director

Microscopy Technician

Research Technician

Location & Contact

BRALM is hosted at the Instituto Biofisika building in the Scientific Park of the Leioa Campus of the UPV/EHU in the metropolitan area of Bilbao.

Address

Instituto Biofisika (CSIC, UPV/EHU)

Scientific Park of the UPV/EHU

Barrio Sarriena s/n

48940 Leioa (Spain)

Phone: (+34) 946 01 3351

e-mail: bralm@csic.es

BRALM Publications

2022-2021

2022


"Ceramide enhances binding of LC3/GABARAP autophagy proteins to cardiolipin-containing membranes"

Varela YR, Iriondo MN, Etxaniz A, Ballesteros U, Montes LR, Goñi FM, Alonso A.

Int J Biol Macromol. 2022 Sep 30;217:748-760.

https://doi.org/10.1016/j.ijbiomac.2022.07.032


"Functional Delineation of a Protein–Membrane Interaction Hotspot Site on the HIV-1 Neutralizing Antibody 10E8"

Insausti S, Garcia-PorrasM, Torralba J, Morillo I, Ramos-Caballero A, de la Arada I, Apellaniz B, Caaveiro JMM, Carravilla P, Eggeling C, Rujas E, Nieva JL.

Int. J. Mol. Sci. 2022, 23(18), 10767

https://doi.org/10.3390/ijms231810767


"LC3 subfamily in cardiolipin-mediated mitophagy: a comparison of the LC3A, LC3B and LC3C homologs."

Iriondo MN, Etxaniz A, Varela YR, Ballesteros U, Hervás JH, Montes LR, Goñi FM, Alonso A.

Autophagy. 2022 Apr 13:1-19.

https://doi.org/10.1080/15548627.2022.2062111


“Plasma membrane effects of sphingolipid-synthesis inhibition by myriocin in CHO cells: a biophysical and lipidomic study.”

Monasterio BG, Jiménez-Rojo N, García-Arribas AB, Riezman H, Goñi FM, Alonso A.

Sci Rep. 2022 Jan 19;12(1):955.

https://doi.org/10.1038/s41598-021-04648-z


2021

“Super-Resolution Microscopy Using a Bioorthogonal-Based Cholesterol Probe Provides Unprecedented Capabilities for Imaging Nanoscale Lipid Heterogeneity in Living Cells."

Lorizate M, Terrones O, Nieto-Garai JA, Rojo-Bartolomé I, Ciceri D, Morana O, Olazar-Intxausti J, Arboleya A, Martin A, Szynkiewicz M, Calleja-Felipe M, Bernardino de la Serna J, Contreras FX.

Small Methods. 2021 Sep;5(9):e2100430.

https://doi.org/10.1002/smtd.202100430


"Focal accumulation of aromaticity at the CDRH3 loop mitigates 4E10 polyreactivity without altering its HIV neutralization profile."

Rujas E, Leaman DP, Insausti S, Carravilla P, García-Porras M, Largo E, Morillo I, Sánchez-Eugenia R, Zhang L, Cui H, Iloro I, Elortza F, Julien JP, Eggeling C, Zwick MB, Caaveiro JMM, Nieva JL.

iScience. 2021 Aug 17;24(9):102987.

https://doi.org/10.1016/j.isci.2021.102987


"CHO/LY-B cell growth under limiting sphingolipid supply: Correlation between lipid composition and biophysical properties of sphingolipid-restricted cell membranes."

Monasterio BG, Jiménez-Rojo N, García-Arribas AB, Riezman H, Goñi FM, Alonso A.

FASEB J. 2021 Jun;35(6):e21657.

https://doi.org/10.1096/fj.202001879RR


“Conformational plasticity underlies membrane fusion induced by an HIV sequence juxtaposed to the lipid envelope.”

de la Arada I, Torralba J, Tascón I, Colom A, Ubarretxena-Belandia I, Arrondo JLR, Apellániz B, Nieva JL.

Sci Rep. 2021 Jan 14;11(1):1278.

https://doi.org/10.1038/s41598-020-80156-w


"Phase-selective staining of model and cell membranes, lipid droplets and lipoproteins with fluorescent solvatochromic pyrene probes."

Sot J, Esnal I, Monasterio BG, León-Irra R, Niko Y, Goñi FM, Klymchenko A, Alonso A.

Biochim Biophys Acta Biomembr. 2021 Jan 1;1863(1):183470.

https://doi.org/10.1016/j.bbamem.2020.183470

2020-2016

2020

"C24:0 and C24:1 sphingolipids in cholesterol-containing, five- and six-component lipid membranes."

González-Ramírez EJ, García-Arribas AB, Sot J, Goñi FM, Alonso A.

Sci Rep. 2020;10(1):14085.

https://doi.org/10.1038/s41598-020-71008-8


"Cholesterol Constrains the Antigenic Configuration of the Membrane-Proximal Neutralizing HIV-1 Epitope."

Torralba J, de la Arada I, Carravilla P, Insausti S, Rujas E, Largo E, Eggeling C, Arrondo JLR, Apellániz B, Nieva JL.

ACS Infect Dis. 2020 Aug 14;6(8):2155-2168.

https://doi.org/10.1021/acsinfecdis.0c00243


"Patches and Blebs: A Comparative Study of the Composition and Biophysical Properties of Two Plasma Membrane Preparations from CHO Cells."

Monasterio BG, Jiménez-Rojo N, García-Arribas AB, Riezman H, Goñi FM, Alonso A.

Int J Mol Sci. 2020 Apr 10;21(7):2643.

https://doi.org/10.3390/ijms21072643


"Photoacoustic effect applied on model membranes and living cells: direct observation with multiphoton excitation microscopy and long-term viability analysis."

Galisteo-González F, Monasterio BG, Gil D, Valle M, Goñi FM.

Sci Rep. 2020 Jan 15;10(1):299.

https://doi.org/10.1038/s41598-019-56799-9


"The Bilayer Collective Properties Govern the Interaction of an HIV-1 Antibody with the Viral Membrane."

Carravilla P, Darré L, Oar-Arteta IR, Vesga AG, Rujas E, de Las Heras-Martínez G, Domene C, Nieva JL, Requejo-Isidro J.

Biophys J. 2020 Jan 7;118(1):44-56.

https://doi.org/10.1016/j.bpj.2019.11.005


2019

"Complex Interplay of Anionic Phospholipid Binding Regulates 3'-Phosphoinositide-Dependent-Kinase-1 Homodimer Activation."

Heras-Martínez GL, Calleja V, Bailly R, Dessolin J, Larijani B, Requejo-Isidro J. A

Sci Rep. 2019 Oct 10;9(1):14527.

https://doi.org/10.1038/s41598-019-50742-8


"Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids."

González-Ramírez EJ, Goñi FM, Alonso A.

Sci Rep. 2019 Sep 16;9(1):13326.

https://doi.org/10.1038/s41598-019-50020-7


"Combining patch-clamping and fluorescence microscopy for quantitative reconstitution of cellular membrane processes with Giant Suspended Bilayers."

Velasco-Olmo A, Ormaetxea Gisasola J, Martinez Galvez JM, Vera Lillo J, Shnyrova AV.

Sci Rep. 2019 May 10;9(1):7255.

https://doi.org/10.1038/s41598-019-43561-4


"Homogeneous and Heterogeneous Bilayers of Ternary Lipid Compositions Containing Equimolar Ceramide and Cholesterol."

González-Ramírez EJ, Artetxe I, García-Arribas AB, Goñi FM, Alonso A.

Langmuir. 2019 Apr 16;35(15):5305-5315.

https://doi.org/10.1021/acs.langmuir.9b00324


"The interaction of lipid-liganded gold clusters (Aurora ™) with lipid bilayers."

Sot J, Mendanha-Neto SA, Busto JV, García-Arribas AB, Li S, Burgess SW, Shaw WA, Gil-Carton D, Goñi FM, Alonso A.

Chem Phys Lipids. 2019 Jan;218:40-46.

https://doi.org/10.1016/j.chemphyslip.2018.11.007


2018

"Molecular Characterization of the Viroporin Function of Foot-and-Mouth Disease Virus Nonstructural Protein 2B."

Gladue DP, Largo E, de la Arada I, Aguilella VM, Alcaraz A, Arrondo JLR, Holinka LG, Brocchi E, Ramirez-Medina E, Vuono EA, Berggren KA, Carrillo C, Nieva JL, Borca MV.

J Virol. 2018 Nov 12;92(23):e01360-18.

https://doi.org/10.1128/JVI.01360-18


"Omega-3 polyunsaturated fatty acids do not fluidify bilayers in the liquid-crystalline state."

De Santis A, Varela Y, Sot J, D'Errico G, Goñi FM, Alonso A.

Sci Rep. 2018 Nov 2;8(1):16240.

https://doi.org/10.1038/s41598-018-34264-3


"Classical Swine Fever Virus p7 Protein Interacts with Host Protein CAMLG and Regulates Calcium Permeability at the Endoplasmic Reticulum."

Gladue DP, Largo E, Holinka LG, Ramirez-Medina E, Vuono EA, Berggren KA, Risatti GR, Nieva JL, Borca MV.

Viruses. 2018 Aug 28;10(9):460.

https://doi.org/10.3390/v10090460


"Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses."

Largo E, Gladue DP, Torralba J, Aguilella VM, Alcaraz A, Borca MV, Nieva JL.

Biochim Biophys Acta Biomembr. 2018 May;1860(5):1015-1021.

https://doi.org/10.1016/j.bbamem.2018.01.012


"Functional Optimization of Broadly Neutralizing HIV-1 Antibody 10E8 by Promotion of Membrane Interactions."

Rujas E, Leaman DP, Insausti S, Ortigosa-Pascual L, Zhang L, Zwick MB, Nieva JL.

J Virol. 2018 Mar 28;92(8):e02249-17.

https://doi.org/10.1128/JVI.02249-17


"Quantifying intracellular equilibrium dissociation constants using single-channel time-resolved FRET."

de Las Heras-Martínez G, Andrieu J, Larijani B, Requejo-Isidro J.

J Biophotonics. 2018 Jan;11(1).

https://doi.org/10.1002/jbio.201600272


2017

"Human ATG3 binding to lipid bilayers: role of lipid geometry, and electric charge."

Hervás JH, Landajuela A, Antón Z, Shnyrova AV, Goñi FM, Alonso A.

Sci Rep. 2017 Nov 15;7(1):15614.

https://doi.org/10.1038/s41598-017-15057-6


"Effects of HIV-1 gp41-Derived Virucidal Peptides on Virus-like Lipid Membranes."

Carravilla P, Cruz A, Martin-Ugarte I, Oar-Arteta IR, Torralba J, Apellaniz B, Pérez-Gil J, Requejo-Isidro J, Huarte N, Nieva JL.

Biophys J. 2017 Sep 19;113(6):1301-1310.

https://doi.org/10.1016/j.bpj.2017.06.061


"Coating Graphene Oxide with Lipid Bilayers Greatly Decreases Its Hemolytic Properties."

Monasterio BG, Alonso B, Sot J, García-Arribas AB, Gil-Cartón D, Valle M, Zurutuza A, Goñi FM.

Langmuir. 2017 Aug 22;33(33):8181-8191.

https://doi.org/10.1021/acs.langmuir.7b01552


"Polyamine-RNA-membrane interactions: From the past to the future in biology."

Acosta-Andrade C, Artetxe I, Lete MG, Monasterio BG, Ruiz-Mirazo K, Goñi FM, Sánchez-Jiménez F.

Colloids Surf B Biointerfaces. 2017 Jul 1;155:173-181.

https://doi.org/10.1016/j.colsurfb.2017.04.005


"Complex Effects of 24:1 Sphingolipids in Membranes Containing Dioleoylphosphatidylcholine and Cholesterol."

García-Arribas AB, González-Ramírez EJ, Sot J, Areso I, Alonso A, Goñi FM.

Langmuir. 2017 Jun 6;33(22):5545-5554.

https://doi.org/10.1021/acs.langmuir.7b00162


"Lipid-modified oligonucleotide conjugates: Insights into gene silencing, interaction with model membranes and cellular uptake mechanisms."

Ugarte-Uribe B, Grijalvo S, Pertíñez SN, Busto JV, Martín C, Alagia A, Goñi FM, Eritja R, Alkorta I.

Bioorg Med Chem. 2017 Jan 1;25(1):175-186.

https://doi.org/10.1016/j.bmc.2016.10.024


2016

"Alteration of a Second Putative Fusion Peptide of Structural Glycoprotein E2 of Classical Swine Fever Virus Alters Virus Replication and Virulence in Swine."

Holinka LG, Largo E, Gladue DP, O'Donnell V, Risatti GR, Nieva JL, Borca MV.

J Virol. 2016 Oct 28;90(22):10299-10308.

https://doi.org/10.1128/JVI.01530-16


"Functional organization of the HIV lipid envelope."

Huarte N, Carravilla P, Cruz A, Lorizate M, Nieto-Garai JA, Kräusslich HG, Pérez-Gil J, Requejo-Isidro J, Nieva JL.

Sci Rep. 2016 Sep 28;6:34190.

https://doi.org/10.1038/srep34190

2015-2011

2015

"Uncoupling PIP2-calmodulin regulation of Kv7.2 channels by an assembly destabilizing epileptogenic mutation."

Alberdi A, Gomis-Perez C, Bernardo-Seisdedos G, Alaimo A, Malo C, Aldaregia J, Lopez-Robles C, Areso P, Butz E, Wahl-Schott C, Villarroel A.

J Cell Sci. 2015 Nov 1;128(21):4014-23.

https://doi.org/10.1242/jcs.176420


"End-product diacylglycerol enhances the activity of PI-PLC through changes in membrane domain structure."

Ahyayauch H, Sot J, Collado MI, Huarte N, Requejo-Isidro J, Alonso A, Goñi FM.

Biophys J. 2015 Apr 7;108(7):1672-1682.

https://doi.org/10.1016/j.bpj.2015.02.020


"Two-photon Laurdan studies of the ternary lipid mixture DOPC:SM:cholesterol reveal a single liquid phase at sphingomyelin:cholesterol ratios lower than 1."

Carravilla P, Nieva JL, Goñi FM, Requejo-Isidro J, Huarte N.

Langmuir. 2015 Mar 10;31(9):2808-17.

https://doi.org/10.1021/la504251u


"Fluorescent polyene ceramide analogues as membrane probes."

Nieves I, Artetxe I, Abad JL, Alonso A, Busto JV, Fajarí L, Montes LR, Sot J, Delgado A, Goñi FM.

Langmuir. 2015 Mar 3;31(8):2484-92.

https://doi.org/10.1021/la505017x


"Interaction of Clostridium perfringens epsilon-toxin with biological and model membranes: A putative protein receptor in cells."

Manni MM, Sot J, Goñi FM.

Biochim Biophys Acta. 2015 Mar;1848(3):797-804.

https://doi.org/10.1016/j.bbamem.2014.11.028


"Histones cause aggregation and fusion of lipid vesicles containing phosphatidylinositol-4-phosphate."

Lete MG, Sot J, Gil D, Valle M, Medina M, Goñi FM, Alonso A.

Biophys J. 2015 Feb 17;108(4):863-871.

https://doi.org/10.1016/j.bpj.2014.12.018


2014

"Biophysical properties of novel 1-deoxy-(dihydro)ceramides occurring in mammalian cells."

Jiménez-Rojo N, Sot J, Busto JV, Shaw WA, Duan J, Merrill AH Jr, Alonso A, Goñi FM.

Biophys J. 2014 Dec 16;107(12):2850-2859.

https://doi.org/10.1016/j.bpj.2014.10.010


"Cholesterol-dependent membrane fusion induced by the gp41 membrane-proximal external region-transmembrane domain connection suggests a mechanism for broad HIV-1 neutralization."

Apellániz B, Rujas E, Carravilla P, Requejo-Isidro J, Huarte N, Domene C, Nieva JL.

J Virol. 2014 Nov;88(22):13367-77.

https://doi.org/10.1128/JVI.02151-14


"N-nervonoylsphingomyelin (C24:1) prevents lateral heterogeneity in cholesterol-containing membranes."

Maté S, Busto JV, García-Arribas AB, Sot J, Vazquez R, Herlax V, Wolf C, Bakás L, Goñi FM.

Biophys J. 2014 Jun 17;106(12):2606-16.

https://doi.org/10.1016/j.bpj.2014.04.054


"Membrane permeabilization induced by sphingosine: effect of negatively charged lipids."

Jiménez-Rojo N, Sot J, Viguera AR, Collado MI, Torrecillas A, Gómez-Fernández JC, Goñi FM, Alonso A.

Biophys J. 2014 Jun 17;106(12):2577-84.

https://doi.org/10.1016/j.bpj.2014.04.038


"Histones and DNA compete for binding polyphosphoinositides in bilayers."

Lete MG, Sot J, Ahyayauch H, Fernández-Rivero N, Prado A, Goñi FM, Alonso A.

Biophys J. 2014 Mar 4;106(5):1092-100.

https://doi.org/10.1016/j.bpj.2014.01.023


"Lamellar gel (lβ) phases of ternary lipid composition containing ceramide and cholesterol."

Busto JV, García-Arribas AB, Sot J, Torrecillas A, Gómez-Fernández JC, Goñi FM, Alonso A.

Biophys J. 2014 Feb 4;106(3):621-30.

https://doi.org/10.1016/j.bpj.2013.12.021


"Membrane binding and insertion of the predicted transmembrane domain of human scramblase 1."

Posada IM, Busto JV, Goñi FM, Alonso A.

Biochim Biophys Acta. 2014 Jan;1838(1 Pt B):388-97.

https://doi.org/10.1016/j.bbamem.2013.09.018


2013

"Double-tailed lipid modification as a promising candidate for oligonucleotide delivery in mammalian cells."

Ugarte-Uribe B, Grijalvo S, Busto JV, Martín C, Eritja R, Goñi FM, Alkorta I.

Biochim Biophys Acta. 2013 Oct;1830(10):4872-84.

https://doi.org/10.1016/j.bbagen.2013.06.013


"Cooperativity between calmodulin-binding sites in Kv7.2 channels."

Alaimo A, Alberdi A, Gomis-Perez C, Fernández-Orth J, Gómez-Posada JC, Areso P, Villarroel A.

J Cell Sci. 2013 Jan 1;126(Pt 1):244-53.

https://doi.org/10.1242/jcs.114082


"Recruitment of a phospholipase C/sphingomyelinase into non-lamellar lipid droplets during hydrolysis of lipid bilayers."

Ibarguren M, Sot J, Montes LR, Vasil AI, Vasil ML, Goñi FM, Alonso A.

Chem Phys Lipids. 2013 Jan;166:12-7.

https://doi.org/10.1016/j.chemphyslip.2012.12.001


2012

"Sphingomyelin organization is required for vesicle biogenesis at the Golgi complex."

Duran JM, Campelo F, van Galen J, Sachsenheimer T, Sot J, Egorov MV, Rentero C, Enrich C, Polishchuk RS, Goñi FM, Brügger B, Wieland F, Malhotra V.

EMBO J. 2012 Dec 12;31(24):4535-46.

https://doi.org/10.1038/emboj.2012.317


"Surface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker."

Aivar P, Fernández-Orth J, Gomis-Perez C, Alberdi A, Alaimo A, Rodríguez MS, Giraldez T, Miranda P, Areso P, Villarroel A.

PLoS One. 2012;7(10):e47263.

https://doi.org/10.1371/journal.pone.0047263


"In situ synthesis of fluorescent membrane lipids (ceramides) using click chemistry."

Garrido M, Abad JL, Alonso A, Goñi FM, Delgado A, Montes LR.

J Chem Biol. 2012 Apr 18;5(3):119-23.

https://doi.org/10.1007/s12154-012-0075-0


"Model systems of precursor cellular membranes: long-chain alcohols stabilize spontaneously formed oleic acid vesicles."

Rendón A, Carton DG, Sot J, García-Pacios M, Montes LR, Valle M, Arrondo JL, Goñi FM, Ruiz-Mirazo K.

Biophys J. 2012 Jan 18;102(2):278-86.

https://doi.org/10.1016/j.bpj.2011.12.026


2011

"Kv7 channels can function without constitutive calmodulin tethering."

Gómez-Posada JC, Aivar P, Alberdi A, Alaimo A, Etxeberría A, Fernández-Orth J, Zamalloa T, Roura-Ferrer M, Villace P, Areso P, Casis O, Villarroel A.

PLoS One. 2011;6(9):e25508.

https://doi.org/10.1371/journal.pone.0025508


"Imaging the early stages of phospholipase C/sphingomyelinase activity on vesicles containing coexisting ordered-disordered and gel-fluid domains."

Ibarguren M, López DJ, Montes LR, Sot J, Vasil AI, Vasil ML, Goñi FM, Alonso A.

J Lipid Res. 2011 Apr;52(4):635-45.

https://doi.org/10.1194/jlr.M012591

2010-2008

2010

"Fluorescence lifetime imaging reveals that the environment of the ATP binding site of myosin in muscle senses force."

Ibanez-Garcia D, Requejo-Isidro J, Webb MR, West TG, French P, Ferenczi MA.

Biophys J. 2010 Oct 6;99(7):2163-9.

https://doi.org/10.1016/j.bpj.2010.07.052


"Cholesterol displaces palmitoylceramide from its tight packing with palmitoylsphingomyelin in the absence of a liquid-disordered phase."

Busto JV, Sot J, Requejo-Isidro J, Goñi FM, Alonso A.

Biophys J. 2010 Aug 9;99(4):1119-28.

https://doi.org/10.1016/j.bpj.2010.05.032


"Dihydrosphingomyelin impairs HIV-1 infection by rigidifying liquid-ordered membrane domains."

Vieira CR, Munoz-Olaya JM, Sot J, Jiménez-Baranda S, Izquierdo-Useros N, Abad JL, Apellániz B, Delgado R, Martinez-Picado J, Alonso A, Casas J, Nieva JL, Fabriás G, Mañes S, Goñi FM.

Chem Biol. 2010 Jul 30;17(7):766-75.

https://doi.org/10.1016/j.chembiol.2010.05.023


"A pore residue of the KCNQ3 potassium M-channel subunit controls surface expression."

Gómez-Posada JC, Etxeberría A, Roura-Ferrer M, Areso P, Masin M, Murrell-Lagnado RD, Villarroel A.

J Neurosci. 2010 Jul 7;30(27):9316-23.

https://doi.org/10.1523/JNEUROSCI.0851-10.2010


2009

"Coexistence of immiscible mixtures of palmitoylsphingomyelin and palmitoylceramide in monolayers and bilayers."

Busto JV, Fanani ML, De Tullio L, Sot J, Maggio B, Goñi FM, Alonso A.

Biophys J. 2009 Nov 18;97(10):2717-26.

https://doi.org/10.1016/j.bpj.2009.08.040


"Calmodulin activation limits the rate of KCNQ2 K+ channel exit from the endoplasmic reticulum."

Alaimo A, Gómez-Posada JC, Aivar P, Etxeberría A, Rodriguez-Alfaro JA, Areso P, Villarroel A.

J Biol Chem. 2009 Jul 31;284(31):20668-75.

https://doi.org/10.1074/jbc.M109.019539


"Calcium inhibits diacylglycerol uptake by serum albumin."

Ahyayauch H, Arana G, Sot J, Alonso A, Goñi FM.

Bio chim Biophys Acta. 2009 Mar;1788(3):701-7.

https://doi.org/10.1016/j.bbamem.2008.11.016


2008


"Cholesterol displacement by ceramide in sphingomyelin-containing liquid-ordered domains, and generation of gel regions in giant lipidic vesicles."

Sot J, Ibarguren M, Busto JV, Montes LR, Goñi FM, Alonso A.

FEBS Lett. 2008 Sep 22;582(21-22):3230-6.

https://doi.org/10.1016/j.febslet.2008.08.016


"Membrane organization and ionization behavior of the minor but crucial lipid ceramide-1-phosphate."

Kooijman EE, Sot J, Montes LR, Alonso A, Gericke A, de Kruijff B, Kumar S, Goñi FM.

Biophys J. 2008 Jun;94(11):4320-30.

https://doi.org/10.1529/biophysj.107.121046


"Calmodulin regulates the trafficking of KCNQ2 potassium channels."

Etxeberria A, Aivar P, Rodriguez-Alfaro JA, Alaimo A, Villacé P, Gómez-Posada JC, Areso P, Villarroel A.

FASEB J. 2008 Apr;22(4):1135-43.

https://doi.org/10.1096/fj.07-9712com

EuroBioImaging Node Candidate

EuroBioImaging Node Candidate Bilbao

BRALM has been selected as a candidate node to join the EuroBioImaging ERIC network.

Acknowledgements

University of the Basque Country
Spanish Research Council (CSIC)
Fundación Biofísica Bizkaia Fundazioa
Ministerio de Ciencia e Innovación
Department of Education of the Basque Government,